Institut Charles Sadron Event

The increasing prevalence of healthcare-associated infections caused by multidrug-resistant pathogens highlights the urgent need for alternative antibacterial strategies, particularly those targeting biofilm formation on medical devices. This PhD thesis investigated the functionalization of enzyme-polyphenol-based coatings developed via layer-by-layer and one-pot deposition. Antimicrobial compounds, such as tannic acid and antibacterial enzymes, were combined to create multifunctional coatings capable of inhibiting bacterial colonization and preventing biofilm formation through diverse mechanisms of action, such as the degradation of the extracellular matrix components, the in situ generation of H2O2, and the release of tannic acid. In parallel, bio-inspired adhesive coatings were developed using Tannic acid or polydopamine to improve the adhesion in plastic metallization, offering a green alternative to toxic treatments. This study presents a promising strategy for designing multifunctional surfaces applied in biomedical and industrial contexts. Key words: biofilm-related infections, antibacterial enzymes, polyphenols, metallization